2. Cell Cycle/DNA Damage Epigenetics Autophagy Anti-infection Apoptosis
  3. HDAC Autophagy Mitophagy Filovirus Apoptosis HPV
  4. Vorinostat

Vorinostat  (Synonyms: 伏立诺他; SAHA; Suberoylanilide hydroxamic acid)

目录号: HY-10221 纯度: 99.62%
COA 产品使用指南

摩尔计算器

Vorinostat (SAHA) 是一种有效的，可口服的 HDAC1，HDAC2，HDAC3 (Class I)，HDAC6 和 HDAC7 (Class II) 和 Class IV (HDAC11) 的抑制剂，对 HDAC1/3 的 ID50 值分别为 10 nM 和 20 nM。Vorinostat 可以诱导细胞凋亡 (apoptosis)。Vorinostat 还是一种人类乳头瘤病毒 (HPV)-18 DNA 扩增的有效抑制剂。

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Vorinostat Chemical Structure

Vorinostat Chemical Structure

CAS No. : 149647-78-9

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥1191
In-stock
250 mg ￥1083
In-stock
500 mg ￥1450
In-stock
1 g ￥2100
In-stock
5 g 现货 询价
10 g   询价  
50 g   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of Vorinostat:

Vorinostat 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 105 篇科研文献

WB

    Vorinostat purchased from MCE. Usage Cited in: RSC Adv., 2018, 8, 17279-17292

    The NH2 cells expressing Tat. All tested cells are treated with SAHA for 24 h. The expression of GFP for tested J-Lat A2 and 2D10 cells is measured by FACS. Whole cell extracts for tested NH1 and NH2 cells are prepared and examined for the luciferase activities.

    Vorinostat purchased from MCE. Usage Cited in: Biochim Biophys Acta Gene Regul Mech. 2018 May 18;S1874-9399(18)30028-2.  [Abstract]

    Jurkat T cells are treated for various times with Vorinostat 1 μM and Chidamide at 0.6 μM. Levels of SALL2 and cleaved PARP are evaluated by Western blot analysis. β-actin is used as a loading control.

    Vorinostat purchased from MCE. Usage Cited in: Nat Commun. 2017 Dec 20;8(1):2207.  [Abstract]

    HCT116 cells are treated with Vorinostat for 24 h, followed by by western blot analysis for the indicated proteins.

    Vorinostat purchased from MCE. Usage Cited in: Mol Psychiatry. 2017 May;22(5):711-723.  [Abstract]

    Oral SAHA administration increases brain plasma membrane calcium ATPase (PMCA) levels, reduces oxidative stress, improves behavioral deficits and prevents neurodegeneration in ASM knockout (ASMko) mice. (a,b) Western blot of hippocampal membrane extracts from wt (a) and ASMko (b) mice orally administered SAHA complexed to HOP-β-CDX (SAHA) or HOP-β-CDX (control), and probed for PMCA and ATP6V1A.

    Vorinostat purchased from MCE. Usage Cited in: Biol Pharm Bull. 2017;40(10):1747-1753.  [Abstract]

    Synergistic Decrease Bcl-2 Expression by the Combination of Vorinostat with BMS-354825 in MCF-7 Cells.

    Vorinostat purchased from MCE. Usage Cited in: Oncotarget. 2017 May 23;8(21):34362-34373.  [Abstract]

    Notch3 protein levels after treatment with SAHA at different concentrations or DMSO (vehicle control), determined by western blot.

    Vorinostat purchased from MCE. Usage Cited in: Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. 15-06-2015.

    SAHA, a histone deacetylase inhibitor that is shown to increase PMCA levels and to enhance calcium clearance in a breast cancer cell model.

    查看 HDAC 亚型特异性产品:

    查看所有亚型
    HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

    查看 Filovirus 亚型特异性产品:

    查看所有亚型
    EBOV MARV ZEBOV

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Vorinostat (SAHA) is a potent and orally active pan-inhibitor of HDAC1, HDAC2 and HDAC3 (Class I), HDAC6 and HDAC7 (Class II) and HDAC11 (Class IV), with ID50 values of 10 nM and 20 nM for HDAC1 and HDAC3, respectively. Vorinostat induces cell apoptosis[1][4]. Vorinostat is also an effective inhibitor of human papillomaviruse (HPV)-18 DNA amplification[7].

    IC50 & Target[1][3][4]

    HDAC1

    10 nM (ID50)

    HDAC3

    20 nM (ID50)

    HDAC2

     

    HDAC7

     

    HDAC11

     

    Autophagy

     

    Mitophagy

     

    体外研究
    (In Vitro)

    Vorinostat efficiently suppresses MES-SA cell growth at a low dosage (3 μM) already after 24 hours treatment. HDACs class I (HDAC2 and 3) as well as class II (HDAC7) are preferentially affected by this treatment. Vorinostat significantly increases p21WAF1 expression and apoptosis in MES-SA cells[1].
    Vorinostat inhibits SK-N-SH and SK-N-Be(2)C with the IC25 values of 1 µM and 0.5 µM, respectively[2].
    Vorinostat is an effective inhibitor of HPV-18 DNA amplification, reduces oncoproteins E6 and E7 activities and triggers apoptosis in HPV-infected, differentiated cells[7].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Vorinostat 相关抗体:
    体内研究
    (In Vivo)

    Vorinostat (50 mg/kg/day) reduces tumor growth by more than 50% in nude mice injected with 5×106 MES-SA cells[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    264.32

    Formula

    C14H20N2O3
    CAS 号
    性状

    固体

    颜色

    White to light yellow

    中文名称

    伏瑞斯特；伏立诺他
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : ≥ 100 mg/mL (378.33 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    * "≥" means soluble, but saturation unknown.

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 3.7833 mL 18.9165 mL 37.8329 mL
    5 mM 0.7567 mL 3.7833 mL 7.5666 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
    ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂： 5% DMSO    40% PEG300    5% Tween-80    50% Saline

      Solubility: ≥ 2.5 mg/mL (9.46 mM); 澄清溶液

    • 方案 二

      请依序添加每种溶剂： 5% DMSO    95% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (9.46 mM); 澄清溶液

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： 20% HP-β-CD in Saline

      Solubility: 3.33 mg/mL (12.60 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    请输入您的动物体内配方组成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。
    您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
    连续给药周期超过半月以上，请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 99.95%

    COA (188 KB) LCMS (94 KB)

    产品使用指南 (1538 KB)
    参考文献
    Cell Assay
    [1]

    Cell lysates are prepared by using RIPA buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS), and the protein concentration is determined by Bio-Rad DC Protein Assay. Protein lysates are separated by SDS-PAGE and transferred to nitrocellulose membrane. Following antibodies and dilutions are used: rabbit anti HDAC1 (1 μg/mL); rabbit anti HDAC2 (1 μg/mL); rabbit anti HDAC3 (9 μg/mL); rabbit anti HDAC7 (3 μg/mL); mouse anti p21WAF1 (0.5 μg/mL). As secondary antibodies, the rabbit anti-mouse and swine anti-rabbit HRP-coupled antibodies at a final concentration of 1 μg/mL. An overnight incubation at 4°C is used for all primary antibodies, followed by washing and 2-hours incubation at RT with secondary antibodies. Specific protein bands are visualized by enhanced chemiluminescence assay. To demonstrate equal loading of protein samples all western blots are probed for β-tubulin.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [1]

    Twelve weeks old male mice (n=14) are anesthetized with Isofluran and 5×106 MES-SA cells are injected subcutaneously into the right flank of the animal. Mice from a control group receives placebo containing 300 μL of empty HOP-β-CD (2-hydroxypropyl-β-cyclodextrin) vesicles. Another group of mice receives vorinostat dissolved in HOP-β-CD at a concentration of 50 mg/kg/day. Both, empty vesicles and vorinostat are administered intraperitoneally, starting on the day 4 after the injection of MES-SA tumor cells. Mice body weight and tumor size (w2 × l × 0.52; measured by caliper) are estimated twice a week. All mice are treated for 21 days and afterwards sacrificed by cervical dislocation. Each tumor is isolated as a whole and different tumor parameters are determined. Finally, tumor slices are cryo preserved and formalin fixed (4%) for further analyses.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    Vorinostat 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.7833 mL 18.9165 mL 37.8329 mL 94.5823 mL
    5 mM 0.7567 mL 3.7833 mL 7.5666 mL 18.9165 mL
    10 mM 0.3783 mL 1.8916 mL 3.7833 mL 9.4582 mL
    15 mM 0.2522 mL 1.2611 mL 2.5222 mL 6.3055 mL
    20 mM 0.1892 mL 0.9458 mL 1.8916 mL 4.7291 mL
    25 mM 0.1513 mL 0.7567 mL 1.5133 mL 3.7833 mL
    30 mM 0.1261 mL 0.6305 mL 1.2611 mL 3.1527 mL
    40 mM 0.0946 mL 0.4729 mL 0.9458 mL 2.3646 mL
    50 mM 0.0757 mL 0.3783 mL 0.7567 mL 1.8916 mL
    60 mM 0.0631 mL 0.3153 mL 0.6305 mL 1.5764 mL
    80 mM 0.0473 mL 0.2365 mL 0.4729 mL 1.1823 mL
    100 mM 0.0378 mL 0.1892 mL 0.3783 mL 0.9458 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Vorinostat149647-78-9SAHA Suberoylanilide hydroxamic acid伏瑞斯特伏立诺他HDACAutophagyMitophagyFilovirusApoptosisHPVHistone deacetylasesMitochondrial AutophagyHuman papillomavirusInhibitorinhibitorinhibit

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